试剂盒 > 酶联免疫试剂盒 > 兔载脂蛋白B100(Apo-B100)ELISA Kit
    • ELISA Kit
    • 兔载脂蛋白B100(Apo-B100)ELISA Kit
    • This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.[provided by RefSeq]
    • UniProtP17165
    • HGNC603
    • RGD2129
    • MGI88052
    • OMIM107730
    • CUSABIOCSB-E09805Rb
  • 澳门银河娱乐官网下载类型:
    ELISA Kit
  • 澳门银河娱乐官网下载名称:

    兔载脂蛋白B100(Apo-B100)ELISA Kit

  • 英文名称:

    Rabbit apolipoprotein B100 (Apo-B100) ELISA Kit

  • 货号:
    CSB-E09805Rb
  • 规格:
    96T
  • 中文价格:
    4200
  • 种属:
    Oryctolagus cuniculus (Rabbit)
  • 待测物名称:
    apolipoprotein B100 (Apo-B100)
  • 缩写:
    Apo-B100
  • 检测范围:
    78 ng/mL-5000 ng/mL
  • 灵敏度:
    19.5 ng/mL
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    50000.092 0.091 0.092
    25000.146 0.138 0.142
    12500.246 0.236 0.241
    6250.375 0.360 0.368
    3120.524 0.539 0.532
    1560.934 0.877 0.906
    781.447 1.334 1.391
    02.432 2.562 2.497
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %96
    Range %90-102
    1:200Average %92
    Range %87-96
    1:400Average %104
    Range %98-108
    1:800Average %85
    Range %80-89
  • 回收率:
    The recovery of rabbit Apo-B100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9893-102
    EDTA plasma (n=4)8782-92
  • 定性/定量:
    quantitative
  • 研究领域:
    Cardiovascular