抗体 > 多克隆抗体 > APEX1 Antibody
  • 澳门银河娱乐官网下载类型:
    Polyclonal Antibody
  • 澳门银河娱乐官网下载名称:

    APEX1 Antibody

  • 货号:
    CSB-PA001900HA01HU
  • 规格:
    ¥1200
  • 别名:
    DNA-(apurinic or apyrimidinic site) lyase (EC 3.1.-.-) (EC 4.2.99.18) (APEX nuclease) (APEN) (Apurinic-apyrimidinic endonuclease 1) (AP endonuclease 1) (APE-1) (REF-1) (Redox factor-1) [Cleaved into: DNA-(apurinic or apyrimidinic site) lyase, mitochondrial], APEX1, APE APE1 APEX APX HAP1 REF1
  • 种属:
    Human
  • 免疫原:
    Recombinant Human DNA-(apurinic or apyrimidinic site) lyase Protein (32-318AA)
  • 宿主:
    Rabbit
  • 种属反应性:
    Human, Mouse
  • 应用范围:
    ELISA, WB, IHC, ChIP; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200
  • 背景:
    Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA.
  • 克隆类型:
    Polyclonal
  • 抗体亚型:
    IgG
  • 纯化方式:
    >95%, Protein G purified
  • 标记方式:
    Non-conjugated
  • 保存缓冲液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 澳门银河娱乐官网下载提供形式:
    Liquid
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 图片:

    Western blot
    All lanes: DNA-(apurinic or apyrimidinic site) lyase antibody at 2ug/ml
    Lane 1: Hela whole cell lysate
    Lane 2: Mouse brain tissue
    Lane 3: MCF-7 whole cell lysate
    Lane 4: HepG2 whole cell lysate
    Lane 5: Raji whole cell lysate
    Lane 6: A549 whole cell lysate
    Lane 7: K562 whole cell lysate
    Lane 8: Mouse liver tissue
    Lane 9: NIH/3T3 whole cell lysate
    Secondary
    Goat polyclonal to Rabbit IgG at 1/15000 dilution
    Predicted band size: 36 kDa
    Observed band size: 36, 34, 21 kDa

    Immunohistochemistry of paraffin-embedded human lungs tissue using CSB-PA001900HA01HU at dilution of 1:100

    Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA001900HA01HU at dilution of 1:100

    Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA001900HA01HU at dilution of 1:100

    Chromatin Immunoprecipitation MCF-7(1.1*106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-APE1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers ( CSB-PP001900HU ) against the MDR1 promoter.

  • 说明书:
  • Accession No.:
  • 研究领域:
    Epigenetics and Nuclear Signaling